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PTH1R/PTHR1 Antibody (734630) [Unconjugated]

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Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos-2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH1R/PTHR1 Monoclonal Antibody (Catalog # MAB5709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTH1R/PTHR1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos-2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
734630
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5709-SP
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PTH1R/PTHR1 Antibody (734630) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PTH1R/PTHR1
Tyr23-Met189
Accession # Q03431
Specificity
Detects human PTH1R/PTHR1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTH1R
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB5709 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTH1R/PTHR1 Antibody (734630) [Unconjugated]

  • MGC138426
  • parathyroid hormone 1 receptorMGC138452
  • parathyroid hormone/parathyroid hormone-related peptide receptor
  • parathyroid hormone/parathyroid hormone-related protein receptor
  • PFE
  • PTH/PTHr receptor
  • PTH/PTHrP type I receptor
  • PTH1 receptor
  • PTH1R
  • PTHR
  • PTHR1
  • PTHRPTHR1parathyroid hormone receptor 1
  • seven transmembrane helix receptor

Background

PTH1R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66-68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca++-release from bone, mediated by osteoclast formation. Mature human PTH1R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1R signaling. Over aa 1-189, human PTH1R shares 88% aa identity with mouse PTH1R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PTH1R/PTHR1 Antibody (734630) [Unconjugated]
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Publications for PTH1R/PTHR1 Antibody (MAB5709)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol PTH1R
Uniprot