Home » PSGL-1/CD162 » PSGL-1/CD162 Antibodies » PSGL-1/CD162 Antibody (031) [CoraFluor™ 1]
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PSGL-1/CD162 Antibody (031) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC
Clone
031
Clonality
Monoclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-90247AF350
Alexa Fluor 405
NBP2-90247AF405
Alexa Fluor 488
NBP2-90247AF488
Alexa Fluor 532
NBP2-90247AF532
Alexa Fluor 594
NBP2-90247AF594
Alexa Fluor 647
NBP2-90247AF647
Alexa Fluor 700
NBP2-90247AF700
Alexa Fluor 750
NBP2-90247AF750
Allophycocyanin
NBP2-90247APC
Biotin
NBP2-90247B
DyLight 350
NBP2-90247UV
DyLight 405
NBP2-90247V
DyLight 488
NBP2-90247G
DyLight 550
NBP2-90247R
DyLight 594
NBP2-90247DL594
DyLight 650
NBP2-90247C
DyLight 680
NBP2-90247FR
DyLight 755
NBP2-90247IR
FITC
NBP2-90247F
HRP
NBP2-90247H
Janelia Fluor 525
NBP2-90247JF525
Janelia Fluor 549
NBP2-90247JF549
Janelia Fluor 585
NBP2-90247JF585
Janelia Fluor 635
NBP2-90247JF635
Janelia Fluor 646
NBP2-90247JF646
Janelia Fluor 669
NBP2-90247JF669
PE
NBP2-90247PE
PerCP
NBP2-90247PCP
Unconjugated
NBP2-90247-100ul
mFluor Violet 450 SE
NBP2-90247MFV450
mFluor Violet 500 SE
NBP2-90247MFV500
mFluor Violet 610 SE
NBP2-90247MFV610
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PSGL-1/CD162 Antibody (031) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human PSGL-1/CD162 (Uniprot#: Q14242-1; Met1-Gly295).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SELPLG
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PSGL-1/CD162 Antibody (031) [CoraFluor™ 1]

  • CD162 antigen
  • CD162
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • PSGL-1cutaneous lymphocyte-associated associated antigen
  • selectin P ligandCLA
  • SELPLG

Background

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SELPLG