![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411381299.jpg "Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRP19 (NP_055317.1). Sequence: AREALATLKPQAGLIVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSV |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRPF19 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PRP19 Antibody (NBP3-35253)Find related products by research area.
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| Gene Symbol | PRPF19 |
![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Rat liver using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411421016.jpg "Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Rat liver using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Human stomach using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411353036.jpg "Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Human stomach using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411334020.jpg "Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of L929 cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411353091.jpg "Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of L929 cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PRP19 Antibody [NBP3-35253] - Western blot analysis of various lysates using PRP19 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411343337.jpg "Western Blot: PRP19 Antibody [NBP3-35253] - Western blot analysis of various lysates using PRP19 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunoprecipitation: PRP19 Antibody [NBP3-35253] - Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug PRP19 antibody. Western blot was performed from the immunoprecipitate using PRP19 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-101220241136460.jpg "Immunoprecipitation: PRP19 Antibody [NBP3-35253] - Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug PRP19 antibody. Western blot was performed from the immunoprecipitate using PRP19 antibody at a dilution of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of U2OS cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411392944.jpg "Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of U2OS cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of H9C2 cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411404087.jpg "Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of H9C2 cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")