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Protamine 2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Protamine 2 Antibody [NBP2-31637] - Analysis in human testis and fallopian tube tissues. Corresponding Protamine 2 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Protamine 2 Antibody [NBP2-31637] - Staining of human testis shows strong nuclear positivity in spermatids.
Immunohistochemistry-Paraffin: Protamine 2 Antibody [NBP2-31637] - Staining of human fallopian tube shows low expression as expected.
Immunohistochemistry-Paraffin: Protamine 2 Antibody [NBP2-31637] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: Protamine 2 Antibody [NBP2-31637] - Staining of human prostate shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Protamine 2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MVRYRVRSLSERSHEVYRQQLHGQEQGHHGQEEQGLSPEHVEVYERTHGQS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Protamine 2 Protein (NBP2-31637PEP)
Publications
Read Publications using
NBP2-31637 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Protamine 2 Antibody

  • cancer/testis antigen family 94, member 2
  • CT94.2
  • FLJ27447
  • protamine 2
  • protamine-2
  • Sperm histone P2
  • Sperm protamine P2

Background

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Protamine 2 Antibody (NBP2-31637)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


Filter By Application
IF/IHC
(1)
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Filter By Species
Human
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-31637 Applications Species
Djureinovic D, Fagerberg L, Hallstrom B et al. The human testis-specific proteome defined by transcriptomics and antibody-based profiling. Mol Hum Reprod 2014-06-01 [PMID: 24598113]
Flannigan R, Mielnik A, Robinson B et al. YBX2 Dysregulation is a Potential Cause for Late Maturation Arrest in Men with Non-Obstructive Azoospermia. bioRxiv 2018-07-09 (IF/IHC, Human) IF/IHC Human

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Bioinformatics

Gene Symbol PRM2
Uniprot