![Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPM1A antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PP2C-alpha-PPM1A-Antibody-Western-Blot-NBP1-32751-img0010.jpg "Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPM1A antibody diluted at 1:500.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PP2C alpha/PPM1A. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPM1A |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7) |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PP2C alpha/PPM1A Antibody (NBP1-32751)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PPM1A |
![Immunocytochemistry/Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPM1A protein stained by PPM1A antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PP2C-alpha-PPM1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32751-img0006.jpg "Immunocytochemistry/Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPM1A protein stained by PPM1A antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP1-32751] - Human colon carcinoma, using PPM1A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/PP2C-alpha-PPM1A-Antibody-Immunohistochemistry-Paraffin-NBP1-32751-img0007.jpg "Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP1-32751] - Human colon carcinoma, using PPM1A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/PP2C-alpha-PPM1A-Antibody-Western-Blot-NBP1-32751-img0008.jpg "Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000")
![Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PP2C-alpha-PPM1A-Antibody-Western-Blot-NBP1-32751-img0009.jpg "Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")