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P-Cadherin Antibody (OTI2D5) - Azide and BSA Free

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Western Blot: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY P-Cadherin.
Immunocytochemistry/ Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY P-Cadherin.
Immunocytochemistry/ Immunofluorescence: P-Cadherin Antibody (OTI2D5) - Azide and BSA Free [NBP2-73267] - Analysis of HeLa cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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P-Cadherin Antibody (OTI2D5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CDH3(NP_001784) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDH3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
Theoretical MW
88.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for P-Cadherin Antibody (OTI2D5) - Azide and BSA Free

  • CAD3
  • cadherin 3, P-cadherin (placental)
  • cadherin 3, type 1, P-cadherin (placental)
  • Cadherin-3
  • CDH3
  • CDHP
  • CDHPcalcium-dependent adhesion protein, placental
  • HJMD
  • PCAD
  • PCadherin
  • P-Cadherin
  • PCADP-cadherin
  • Placental cadherin

Background

The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. R-cadherin (for retinal-cadherin, also designated cadherin-4), which was first identified in the retina of chicken, has been shown to be involved in the development of striated muscle and potentially epithelia in addition to its involvement in retinal development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDH3