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NCAPG2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: NCAPG2 Antibody [NBP1-86633] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human tonsil shows moderate nuclear positivity in germinal center and non-germinal center cells.
Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human prostate shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human skeletal muscle shows weak to moderate nuclear positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NCAPG2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QVGHILELVDNWLPTEHAQAKSNTASKGRVQIHDTRPVKPELALVYIEYLLTHPKNRECLLSAPRKKLNHLLKALETSKADLESLLQTPGGKPRGFSEAAAPRAFGLHCRLSIHLQH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCAPG2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NCAPG2 Protein (NBP1-86633PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NCAPG2 Antibody

  • Chromosome-associated protein G2
  • FLJ20311
  • Leucine zipper protein 5CAP-G2hCAP-G2condensin-2 complex subunit G2
  • LUZP5
  • more than blood homolog
  • MTB
  • Non-SMC condensin II complex subunit G2
  • non-SMC condensin II complex, subunit G2

Background

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NCAPG2
Entrez
Uniprot