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NAPRT1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87244 antibody. Corresponding NAPRT RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human colon, kidney, lymph node and urinary bladder using Anti-NAPRT antibody NBP1-87244 (A) shows similar protein ...read more
Independent Antibodies: Western Blot: NAPRT1 Antibody [NBP1-87244] - Analysis using Anti-NAPRT antibody NBP1-87244 (A) shows similar pattern to independent antibody NBP1-87243 (B).
Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human placenta shows moderate cytoplasmic positivity in tropoblastic cells.
Genetic Strategies: Knockdown Validated: Rabbit Polyclonal NAPRT1 Antibody [NBP1-87244] - Immunoblot using primary cultured rat cardiomyocyte with NAPRT1 knockdown using the siRNA. Image from a verified customer ...read more

Product Details

Summary
Reactivity Hu, Mu, ChHa, RtSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NAPRT1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PAFFEHLRALDCSEVTVRALPEGSLAFPGVPLLQVSGPLLVVQLLETPLLCLVSYASLVATNAARLRLIAGP
Predicted Species
Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAPRT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated Validated for Knockdown from a verified customer review.
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NAPRT1 Protein (NBP1-87244PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-87244 in the following applications:

Publications
Read Publication using
NBP1-87244 in the following applications:

  • WB
    1 publication

Reactivity Notes

Chinese Hamster, Mouse reactivity reported in scientific literature (PMID: 24204194).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NAPRT1 Antibody

  • EC 2.4.2.11
  • FHA-HIT-interacting protein
  • FHIP
  • NAPRTase
  • nicotinate phosphoribosyltransferase domain containing 1
  • Nicotinate phosphoribosyltransferase domain-containing protein 1
  • nicotinate phosphoribosyltransferase
  • nicotinic acid phosphoribosyltransferase
  • PP3856

Background

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NAPRT1 Antibody (NBP1-87244)(1)

We have publications tested in 3 confirmed species: Human, Mouse, Chinese Hamster.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
Mouse
(1)
Chinese Hamster
(1)
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Review for NAPRT1 Antibody (NBP1-87244) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-87244:
Filter by Applications
KD
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Shinichi Oka
KD Rat 11/28/2023
View

Summary

ApplicationKnockdown Validated
Sample Testedprimary neonatal ventricular cardiomyocytes
SpeciesRat
LotB69496

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NAPRT1 Antibody (NBP1-87244) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
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1
0

Shinichi Oka
11/28/2023
Application: KD
Species: Rat

Bioinformatics

Gene Symbol NAPRT
Entrez
Uniprot