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Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody

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Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NB100-41399] - Staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NB100-41399] - Staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Mu, Rt, Hu, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody Summary

Immunogen
Peptide with sequence C-DKRRWRKIPKRPGSVH corresponding to C-Terminus according to NP_000729.2.
Predicted Species
Human (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHRM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx 50 kDa band observed in mouse heart lysates (calculated MW of 51.4 kDa according to NP_000729.2).
Control
Muscarinic Acetylcholine Receptor M1/CHRM1 Overexpression Lysate
Publications
Read Publications using
NB100-41399 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21389686).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody

  • cholinergic receptor, muscarinic 1
  • CHRM1
  • HM1
  • M1
  • M1R
  • mAChR M1
  • MGC30125
  • muscarinic acetylcholine receptor M1

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody (NB100-41399)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

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Control Lysate(s)

Muscarinic Acetylcholine Receptor M1/CHRM1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHRM1
Entrez
Uniprot