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MUC4 Antibody (MUC4/3105)

Images

 
Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC4 Antibody (MUC4/3105).
Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC4 Antibody (MUC4/3105).
Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC4 Antibody (MUC4/3105).
Protein Array: MUC4 Antibody (MUC4/3105) [NBP2-79732] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MUC4 Antibody (MUC4/3105) Z- and S- Score: The Z-score represents the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, MA
Clone
MUC4/3105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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MUC4 Antibody (MUC4/3105) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79867)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment (around aa 1730-1864) of human MUC4 protein (exact sequence is proprietary) (Uniprot: Q99102)
Localization
Cytoplasmic
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
235 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC4 Antibody (MUC4/3105)

  • Ascites sialoglycoprotein
  • ASGP
  • HSA276359
  • MUC4
  • MUC-4
  • mucin 4, cell surface associated
  • mucin 4, tracheobronchial
  • mucin-4
  • Pancreatic adenocarcinoma mucin
  • Testis mucin
  • Tracheobronchial mucin

Background

MUC4 is a constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, and is a highly glycosylated proteins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. MUC4 antibody studies have shown that MUC4 encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of MUC4 have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. MUC4 is expressed in a wide variety of carcinomas making MUC4 antibodies a key cancer research tool.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Blogs on MUC4.

MUC4 (Mucin-4)
Mucus is the viscous secretion that covers epithelial surfaces (trachea, colon, and cervix) and consists of twenty highly glycosylated proteins called mucins. The mucin family all are high-molecular weight proteins with oligosaccharides attached to th...  Read full blog post.

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Bioinformatics

Gene Symbol MUC4
Uniprot