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Mineralocorticoid R/NR3C2 Antibody

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Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640] - Antibody Titration: 1. 25 ug/ml Positive control: A172 cell lysate NR3C2 is supported by BioGPS gene expression data to be expressed in A172.
Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X ...read more
Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP1-70640] - Simple Western lane view shows lysates of A172. A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 107 kDa (as indicated) using ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Mineralocorticoid R/NR3C2 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to NR3C2(nuclear receptor subfamily 3, group C, member 2) The peptide sequence was selected from the middle region of NR3C2. Peptide sequence RRKNCPACRLQKCLQAGMNLGARKSKKLGKLKGIHEEQPQQQQPPPPPPP. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR3C2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Simple Western
  • Western Blot 1.0 ug/ml
Theoretical MW
107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-70640 in the following applications:

  • WB
    1 publication

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23592886).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Mineralocorticoid R/NR3C2 Antibody

  • FLJ41052
  • MGC133092
  • Mineralocorticoid R
  • mineralocorticoid receptor 1
  • mineralocorticoid receptor delta
  • MineralocorticoidR
  • MLR
  • MLRmineralocorticoid receptor
  • MRMCRaldosterone receptor
  • NR3C2
  • NR3C2VIT
  • Nuclear receptor subfamily 3 group C member 2
  • nuclear receptor subfamily 3, group C, member 2

Background

NR3C2 is a receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. It binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mineralocorticoid R/NR3C2 Antibody (NBP1-70640)(1)

We have publications tested in 1 confirmed species: Primate.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol NR3C2
Entrez
Uniprot