![Block/Neutralize: MERS-CoV Spike Protein Antibody (723) [NBP2-90336] - MERS-CoV Spike Protein antibody (723) measured by its ability to inhibit infection of Caco-2 cells induced by MERS-CoV pseudovirus. The ED50 for this effect is 2.5-11 ng/mL.](http://images.novusbio.com/fullsize/MERS-CoV-Spike-Protein-Antibody-723-Block-Neutralize-NBP2-90336-img0001.jpg "Block/Neutralize: MERS-CoV Spike Protein Antibody (723) [NBP2-90336] - MERS-CoV Spike Protein antibody (723) measured by its ability to inhibit infection of Caco-2 cells induced by MERS-CoV pseudovirus. The ED50 for this effect is 2.5-11 ng/mL.")
| Reactivity | VSpecies Glossary |
| Applications | B/N |
| Clone | 723 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant MERS-CoV (Accession#: AFS88936.1; Met1-Trp1297). |
| Specificity | Has cross-reactivity in ELISA with: MERS-CoV Spike Protein (aa 1-1297) MERS-CoV Spike Protein S1 (aa 1-725) MERS-CoV Spike Protein RBD (aa 367-606) No cross-reactivity in ELISA with: MERS-CoV Spike Protein S2 (aa 726-1296) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | S |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Microneutralization: Neutralization of MERS-CoV pseudovirus by NBP2-90336. Positive Control:293T/DPP4 cells were infected with MERS-CoV pseudovirus; Negative Control:293T/DPP4 cells without MERS-CoV pseudovirus; NBP2-90336: MERS-CoV pseudovirus infection in 293T/DPP4 cells was inhibited by NBP2-90336. Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | S |
| Uniprot |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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