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MDM2/HDM2 Antibody (197702)

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U2OS human osteosarcoma cell line was untreated (-) or treated (+) with proteasome inhibitor LLnV for 1 hour. MDM2 was immunoprecipitated from 500 µg of cell lysate following incubation with 2-3 µg Mouse Anti-Human MDM2 Monoclonal Antibody (Catalog # MAB1244) for 1 hour on ice. MDM2-antibody complexes were absorbed using goat anti-mouse agarose (Sigma). Immunoprecipitated MDM2 was detected by Western blot using 1 µg/mL Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244). View our recommended buffer recipes for immunoprecipitation.
U2OS human osteosarcoma cell line was untreated (-) or treated (+) with proteasome inhibitor LLnV for 1 hour. MDM2 was immunoprecipitated from 500 µg of cell lysate following incubation with 2-3 µg Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP
Clone
197702
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB1244-SP
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MDM2/HDM2 Antibody (197702) Summary

Immunogen
E. coli-derived recombinant human MDM2/HDM2
Asn3-Pro491
Accession # Q00987
Specificity
Detects human MDM2/HDM2.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MDM2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2.5 ug/500 ug cell lysate
Reviewed Applications
Read 1 Review rated 5
using
MAB1244 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MDM2/HDM2 Antibody (197702)

  • ACTFS
  • HDM2
  • HDMX
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • MDM2

Background

MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally upregulates the expression of its own inhibitor, MDM2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDM2/HDM2 Antibody (MAB1244) (0)

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Review for MDM2/HDM2 Antibody (MAB1244) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1244:
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(1)
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Images Ratings Applications Species Date Details
Western Blot MDM2/HDM2 MAB1244
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 03/09/2016
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Summary

ApplicationWestern Blot
Sample TestedHuman medulloblastoma cells
SpeciesHuman
LotKRP011602A

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Western Blot MDM2/HDM2 MAB1244
Enlarge
 Verified Customer
03/09/2016
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol MDM2
Uniprot