![Immunohistochemistry: Laminin beta 1 Antibody [NB120-6571] - Tissue: skin. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Laminin at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-Immunohistochemistry-NB120-6571-img0002.jpg "Immunohistochemistry: Laminin beta 1 Antibody [NB120-6571] - Tissue: skin. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Laminin at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. |
| Immunogen | Laminin beta 1 Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin. (Uniprot: P07942) |
| Localization | Extracellular. Found in the basement membranes. |
| Marker | Schwann Cell Marker |
| Specificity | Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminin beta 1s and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin beta 1 antibodies with other human serum proteins or non-Laminin beta 1 extracellular matrix proteins is negligible. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LAMB1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by IHC and was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. Anti-Laminin (Human) (RABBIT) Antibody was also assayed against 1.0 ug of Laminin in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Specific conditions should be optimized by researcher. |
| Storage | Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles. |
| Buffer | 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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