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Integrin beta 2/CD18 Antibody (212701) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
212701
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1730U-100UG
Alexa Fluor 405
FAB1730V-100UG
Alexa Fluor 488
FAB1730G-100UG
Alexa Fluor 532
FAB1730X
Alexa Fluor 594
FAB1730T-100UG
Alexa Fluor 647
FAB1730R-100UG
Alexa Fluor 700
FAB1730N-100UG
Alexa Fluor 750
FAB1730S-100UG
Allophycocyanin/Cy7
FAB1730APCCY7
Biotin
FAB1730B
DyLight 350
FAB1730D
DyLight 405
FAB1730E
DyLight 488
FAB1730K
DyLight 550
FAB1730L
DyLight 594
FAB1730M
DyLight 650
FAB1730W
DyLight 680
FAB1730Y
DyLight 755
FAB1730Z
HRP
FAB1730H
Janelia Fluor 525
FAB1730JF525
Janelia Fluor 585
FAB1730JF585
Janelia Fluor 635
FAB1730JF635
Janelia Fluor 646
FAB1730J
Janelia Fluor 669
FAB1730JF669
PE/Atto594
FAB1730PEATT594
PE/Cy5.5
FAB1730PECY55
PE/Cy7
FAB1730PECY7
PerCP
FAB1730C
Phycoerythrin
FAB1730P
Unconjugated
MAB1730
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1730
Carrier Free
FAB1730P
Carrier Free
MAB1730
Carrier Free
MAB1730-SP
Carrier Free
FAB1730V-100UG
Carrier Free
FAB1730U-100UG
Carrier Free
FAB1730T-100UG
Carrier Free
FAB1730S-100UG
Carrier Free
FAB1730R-100UG
Carrier Free
FAB1730N-100UG
Carrier Free
FAB1730G-100UG
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Integrin beta 2/CD18 Antibody (212701) [Janelia Fluor® 549] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin  beta 2/CD18
Gln23-Asn700
Accession # AAA59490
Specificity
Detects human Integrin beta 2/CD18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin  beta 1 or recombinant mouse Integrin  alpha 5 is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB2
Purity
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Integrin beta 2/CD18 Antibody (212701) [Janelia Fluor® 549]

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Background

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ITGB2