![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80818-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively. Image from the standard format of this antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP, B/N, CyTOF-ready |
| Clone | P4C10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | HT1080 cells human fibrosarcoma |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ITGB1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For ICC/IF ,methanol fixation is recommended. The P4C10 antibody inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. |
| Theoretical MW | 88 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Integrin beta 1/CD29 Antibody (NBP2-80818)Find related products by research area.
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Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ITGB1 |
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0004.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0002.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0003.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0005.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")