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Integrin alpha L/CD11a Antibody

Images

 
Western Blot: Integrin alpha L/CD11a Antibody [NBP1-51967] - (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967] - (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967] - (3.8ug/ml) Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Integrin alpha L/CD11a Antibody Summary

Immunogen
Peptide with sequence C-YQVRIQPSIHDHN corresponding to internal region according to NP_002200.2, NP_001107852.1.
Epitope
C-YQVRIQPSIHDHN
Specificity
This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITGAL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4-6 ug/ml
  • Peptide ELISA 1:16000
  • Western Blot 0.5-2 ug/ml
Application Notes
WB: Approx. 200 kDa band observed in human spleen and tonsil lysates (calculated MW of 129 kDa band according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. IHC-P: Human liver shows selected staining of cells within aggregated splenocytes of the white pulp.
Control
Blood Leukocyte Whole Cell Lysate
Human Appendix Whole Tissue Lysate (Adult Whole Normal)
Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NBP1-51967.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Integrin alpha L/CD11a Antibody

  • CD11 antigen-like family member A
  • CD11a antigen
  • CD11a
  • CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
  • Integrin alpha L
  • integrin alpha-L
  • integrin gene promoter
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
  • ITGAL
  • Leukocyte adhesion glycoprotein LFA-1 alpha chain
  • Leukocyte function-associated molecule 1 alpha chain
  • LFA-1 alpha
  • LFA-1
  • LFA1A
  • LFA-1A
  • Ly-15
  • Ly-21
  • lymphocyte function-associated antigen 1

Background

CD11a is a 180 kD glycoprotein, also known as alpha-L integrin, LFA-1 alpha, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The 2D7 antibody can block CD11a-mediated cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha L/CD11a Antibody (NBP1-51967)(1)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol ITGAL
Entrez
Uniprot