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IGSF8/CD316 Antibody [Biotin]

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IGSF8/CD316 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Mouse IGSF8/CD316 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IGSF8/CD316 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Mouse IGSF8/CD316 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3117) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF3117
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3117
Carrier Free
AF3117
Carrier Free
AF3117-SP
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IGSF8/CD316 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGSF8/CD316
Ala25-Thr577
Accession # NP_536344
Specificity
Detects mouse IGSF8/CD316 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGSF8
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGSF8/CD316 Antibody [Biotin]

  • CD316 antigen
  • CD316
  • CD81P3
  • CD81P3LIR-D1
  • EWI2
  • EWI-2
  • EWI2CD81 partner 3
  • Glu-Trp-Ile EWI motif-containing protein 2
  • IGSF8
  • immunoglobulin superfamily, member 8
  • KCT4
  • KCT-4
  • Keratinocytes-associated transmembrane protein 4
  • PGRL
  • PGRLimmunoglobulin superfamily member 8

Background

IGSF8, also known as PGRL (PG regulatory-like protein), KASP (KAI/CD82 associated protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widely expressed transmembrane adhesion protein. It interacts with beta 1 Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8 contains four extracellular Ig-like domains. IGSF8 overexpression in transformed cells inhibits cell migration and suppresses cancer metastatic potential. Mouse and human IGSF8 share 90% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IGSF8/CD316
Antibody [Biotin]
BAF3117
IGSF8/CD316 Antibody [Biotin]
Species: Mu
Applications: WB, Flow, ICC/IF

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Bioinformatics

Gene Symbol IGSF8
Uniprot