Porcine ICAM-1/CD54 ELISA Kit (Colorimetric) Summary
Description |
Assay time: 3 hours |
Standard Curve Range |
1.56 - 100 ng/mL |
Sensitivity |
0.65 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 12 |
Intra-Assay |
%CV < 10 |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ICAM1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated ready to use 96-well strip plate
- Standard Diluent
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30x concentrate)
|
Alternate Names for Porcine ICAM-1/CD54 ELISA Kit (Colorimetric)
Background
ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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