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Human Phospho-MSPR/Ron DuoSet IC ELISA, 2 Plate

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MDA-MB-453 human breast cancer cells were treated with 400 ng/mL recombinant human MSP (Catalog # 352-MS) for five minutes to induce tyrosine phosphorylation of MSP R. Serial dilutions of lysates were analyzed by (A) IP-Western Blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-MSP R monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-MSP R. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Human phospho-MSP R can be detected in this DuoSet® IC ELISA by using approximately 6-12 times less lysate than is needed for a conventional IP-Western Blot.
MDA-MB-453 human breast cancer cells were treated with 400 ng/mL recombinant human MSP (Catalog # 352-MS) for five minutes to induce tyrosine phosphorylation of MSP R. Serial dilutions of lysates were analyzed by (A) ...read more
MDA-MB-453 human breast cancer cells were untreated or treated with 400 ng/mL recombinant human MSP for five minutes. ELISA and IP-Western Blot (inset) analyses were performed using 100 μg and 400 μg of lysate, ...read more
MDA-MB-453 human breast cancer cells were treated with 400 ng/mL recombinant human (rh) MSP for five minutes. The indicated amounts of recombinant extracellular domains of human MSP R, human HGF R/Fc Chimera (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
DYC1947-5
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Human Phospho-MSPR/Ron DuoSet IC ELISA, 2 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
MST1R

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publication using DYC1947-2.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-MSPR/Ron DuoSet IC ELISA, 2 Plate

  • CD136 antigen
  • CD136
  • CDw136c-met-related tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.1
  • macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
  • MSP R
  • MSP receptor
  • MSPR
  • MST1R
  • p185-Ron
  • Protein-tyrosine kinase 8
  • PTK8 protein tyrosine kinase 8
  • PTK8
  • Ron
  • RONmacrophage-stimulating protein receptor
  • soluble RON variant 1
  • soluble RON variant 2
  • soluble RON variant 3
  • soluble RON variant 4

Background

Macrophage stimulating protein receptor (MSPR), encoded by the human RON and the mouse Stk genes, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea. This family of receptors is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular a chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity.

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Human
Phospho-MSPR/Ron DuoS ...
DYC1947-2
Human Phospho-MSPR/Ron DuoSet IC ELISA, 2 Plate
Species: Hu
Applications: ELISA

Publications for MSPR/Ron (DYC1947-2)(1)

We have publications tested in 1 confirmed species: Human.


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Bioinformatics

Gene Symbol MST1R