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HSP60 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 at approximately 60 ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
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HSP60 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HSP60
Met1-Phe573
Accession # P10809
Specificity
Detects human, mouse, and rat HSP60 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPD1
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 0.5 - 1 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF1800 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP60 Antibody [Unconjugated]

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • GROEL
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13

Background

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat-shock or other cellular stress.  Full length human HSP60 is 98% identical to mouse and rat HSP60.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP60 Antibody (AF1800)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Simple Western, Western Blot.


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Simple Western
(1)
Western Blot
(1)
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Filter By Species
Human
(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSP60 Antibody (AF1800) (0)

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Secondary Antibodies

 

Isotype Controls

Additional HSP60 Products

Blogs on HSP60.

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Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat ...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Bioinformatics

Gene Symbol HSPD1
Uniprot