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HSF4 Antibody (2E7) - BSA Free

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Western Blot: HSF4 Antibody (2E7) [NBP2-37499] - Western blot analysis using HSF4 mAb against human HSF4 (AA: 245-411) recombinant protein. (Expected MW is 42.9 kDa)
Flow Cytometry: HSF4 Antibody (2E7) [NBP2-37499] - Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
ELISA: HSF4 Antibody (2E7) [NBP2-37499] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
2E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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HSF4 Antibody (2E7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human HSF4 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSF4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSF4 Antibody (2E7) - BSA Free

  • ATHSF4
  • AT-HSFB1
  • cataract, Marner
  • CTM
  • heat shock factor protein 4
  • heat shock transcription factor 4CTM
  • hHSF4
  • HSF 4
  • HSF4
  • HSFB1
  • HSTF 4

Background

HSF4 is a gene which codes for a DNA-binding protein that binds to heat shock promoter elements and is expressed in the heart, skeletal muscle, eyes and brain, with an isoform with a length of 492 amino acids and a weight of approximately 53 kDa which activates transcription, and a shorter isoform with a length of 462 amino acids and a weight of approximately 50 kDa that represses transcription. Current studies are being done on several disorders and diseases relating to this gene including cataract, otitis media, cervical adenitis, Scheuermann's disease, Ritter's disease, and impetigo. HSF4 has also been shown to have interactions with CDK2AP2, NUDT21, C5orf54, MAPK1, and DUSP26.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSF4