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Histone Deacetylase 4/HDAC4 Antibody [CoraFluor™ 1]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Ch, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22151AF350
Alexa Fluor 405
NBP2-22151AF405
Alexa Fluor 488
NBP2-22151AF488
Alexa Fluor 532
NBP2-22151AF532
Alexa Fluor 594
NBP2-22151AF594
Alexa Fluor 647
NBP2-22151AF647
Alexa Fluor 700
NBP2-22151AF700
Alexa Fluor 750
NBP2-22151AF750
Allophycocyanin
NBP2-22151APC
Biotin
NBP2-22151B
DyLight 350
NBP2-22151UV
DyLight 405
NBP2-22151V
DyLight 488
NBP2-22151G
DyLight 550
NBP2-22151R
DyLight 594
NBP2-22151DL594
DyLight 650
NBP2-22151C
DyLight 680
NBP2-22151FR
DyLight 755
NBP2-22151IR
FITC
NBP2-22151F
HRP
NBP2-22151H
Janelia Fluor 525
NBP2-22151JF525
Janelia Fluor 549
NBP2-22151JF549
Janelia Fluor 585
NBP2-22151JF585
Janelia Fluor 635
NBP2-22151JF635
Janelia Fluor 646
NBP2-22151JF646
Janelia Fluor 669
NBP2-22151JF669
PE
NBP2-22151PE
PerCP
NBP2-22151PCP
Unconjugated
NBP2-22151-0.1ml
mFluor Violet 450 SE
NBP2-22151MFV450
mFluor Violet 500 SE
NBP2-22151MFV500
mFluor Violet 610 SE
NBP2-22151MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22151-0.1ml
BSA Free
NBP2-22151-0.025ml
BSA Free
NBP2-22151-0.1ml
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Histone Deacetylase 4/HDAC4 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A synthetic peptide made to a C-terminal portion of the human HDAC4 protein (between residues 1000-1084). [UniProt P56524]
Localization
Nucleus and cytoplasm.
Predicted Species
Chicken (94%), Zebrafish (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Histone Deacetylase 4/HDAC4 Antibody [CoraFluor™ 1]

  • HA6116
  • HD4EC 3.5.1.98
  • HDAC4
  • HDAC-A
  • HDACABDMR
  • Histone Deacetylase 4
  • histone deacetylase A
  • KIAA0288AHO3

Background

Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HDAC4