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HGS Antibody (OTI3H7) - Azide and BSA Free

Images

 
Western Blot: HGS Antibody (OTI3H7) - Azide and BSA Free [NBP2-71418] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGS .
Immunocytochemistry/ Immunofluorescence: HGS Antibody (OTI3H7) - Azide and BSA Free [NBP2-71418] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HGS.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
OTI3H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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HGS Antibody (OTI3H7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human HGS(NP_004703) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HGS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:4000
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HGS Antibody (OTI3H7) - Azide and BSA Free

  • FLJ39878
  • hepatocyte growth factor-regulated tyrosine kinase substrate
  • Hrs
  • HRSFLJ11421
  • human growth factor-regulated tyrosine kinase substrate
  • Protein pp110
  • Vps27
  • ZFYVE8

Background

HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early end

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HGS Antibody (NBP2-71418) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HGS