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Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free

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Western Blot: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Guanylate kinase (Right lane) cDNA for 48 hrs ...read more
Immunohistochemistry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - Staining of paraffin-embedded Human pancreas tissue using anti-Guanylate kinase mouse monoclonal antibody.
Flow Cytometry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more
Immunohistochemistry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Guanylate kinase mouse monoclonal antibody.
Immunohistochemistry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Guanylate kinase mouse monoclonal antibody.
Immunohistochemistry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Guanylate kinase mouse monoclonal antibody.
Immunohistochemistry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - Staining of paraffin-embedded Human prostate tissue using anti-Guanylate kinase mouse monoclonal antibody.
Flow Cytometry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - Analysis of Hela cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free [NBP2-70869] - Analysis of Jurkat cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI4A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human GUK1 (NP_000849) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GUK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Guanylate kinase Antibody (OTI4A8) - Azide and BSA Free

  • ATP:GMP Phosphotransferase
  • Deoxyguanylate Kinase
  • EC 2.7.4.8
  • FLJ42686
  • FLJ43710
  • GMK
  • GMP Kinase
  • Guanosine monophosphate Kinase
  • guanylate kinase 1
  • Guanylate Kinase
  • GUK1

Background

Essential for recycling GMP and indirectly, cGMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GUK1