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GR/NR3C1 Antibody (C.15200010)

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Western Blot: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10^6 Raji or Molt cells were analyzed by Western blot using ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary ...read more
Immunohistochemistry: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Immunoreactivity of the monoclonal antibody against hGR in rat CA1 neurons of hippocampus. The antibody was used at 2.5 ug/mL.
Chromatin Immunoprecipitation: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - ChIP assays were performed using HeLa cells, the monoclonal antibody directed against GR and optimized PCR primer sets for qPCR. The cells ...read more
Immunoprecipitation: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Immunoprecipitation using the antibody against hGR. The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in ...read more
ELISA: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Sandwich ELISA The specificity of the antibody directed against hGR was assessed by sandwich ELISA. Figure A: schematic representation of the sandwich ELISA with the ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, GS
Clone
C.15200010
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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GR/NR3C1 Antibody (C.15200010) Summary

Immunogen
The exact immunogen is propietary information.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NR3C1
Purity
ammonium sulphate purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/IP
  • Chromatin Immunoprecipitation Sequencing 5ug/ChIP
  • ELISA 0.5 ug/mL
  • Flow Cytometry 0.5 ug/mL
  • Gel Supershift Assay 5 ug/mL
  • Immunocytochemistry/ Immunofluorescence 2.5 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunoprecipitation 5 ug/IP
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
ammonium sulphate purified

Alternate Names for GR/NR3C1 Antibody (C.15200010)

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GR/NR3C1 Antibody (NBP2-59202) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NR3C1