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GPR64 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining in human epididymis and endometrium tissues using NBP1-84906 antibody. Corresponding ADGRG2 RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining of human endometrium, epididymis, liver and skeletal muscle using Anti-ADGRG2 antibody NBP1-84906 (A) shows similar ...read more
Biological Strategies: Western Blot: GPR64 Antibody [NBP1-84906] - Expression of selected genes in BCC subsets. Western blots for GPR64, TMEM98, FAT4, RAP1A and FN1 using whole cell extracts expression from ...read more
Flow Cytometry: GPR64 Antibody [NBP1-84906] - Characterization of BCC subsets. MDA-MB-231, stably transfected with Oct4-GFP (left panel), was labeled with PE-anti-TMEM-98, -GPR64 and -FAT4 and analyzed by flow cytometry ...read more
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining of human epididymis shows strong positivity in microvilli in glandular cells.
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Expression of selected genes in BCC subsets. (A) Real time PCR for GPR64, FAT4, TMEM98, ALOX5 AP, DUSP4, RAP1A & FN1. The RNA was isolated from Oct4hi, Octmed & Oct4low subsets from MDA-MB-231 stably transfected with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GPR64 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SPTVSAPANVNTTSAPPVQTDIVNTSSISDLENQVLQMEKALSLGSLEPNLAGEMINQVSRLLHSPPDMLAPLAQRLLKVVDDIGLQLNFSNTTISLTSPSLALAVIRVNASSFNTTTFVAQDPANLQVSLETQAPE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADGRG2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in WB reported in scientific literature (PMID: 29321622).
Control Peptide
GPR64 Protein (NBP1-84906PEP)
Publications
Read Publication using
NBP1-84906 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GPR64 Antibody

  • EDDM6
  • epididymis-specific (seven transmembrane family)
  • G protein-coupled receptor 64
  • GPR64
  • HE6
  • MGC104454
  • MGC138738
  • TM7LN2

Background

GPR64 is an Orphan-B GPCR with an unknown ligand. HE6 is endogenously cleaved into two pieces at the GPCR proteolytic site (GPS) located adjacent to the first transmembrane helix as a mechanism to compartmentalize the cell adhesion and GPCR activation functions. GPR64 has been reported in the epididymis. ESTs have been isolated from embryo, kidney, placenta, skeletal muscle, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPR64 Antibody (NBP1-84906)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ADGRG2