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Glutathione Peroxidase 5 Antibody

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.74 mg/ml

Order Details

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Glutathione Peroxidase 5 Antibody Summary

Immunogen
Immunized with recombinant Rat Glutathione Peroxidase 5 (Uniprot#: P30710; Thr22-Ile221 (Uniprot#: P30710)
Clonality
Polyclonal
Host
Rabbit
Gene
GPX5
Purity
Antigen and protein A Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5-20ug/mL
  • Immunohistochemistry 5-20ug/mL
  • Western Blot 0.5-2ug/mL

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH7.4, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
0.74 mg/ml
Purity
Antigen and protein A Affinity-purified

Alternate Names for Glutathione Peroxidase 5 Antibody

  • EC 1.11.1
  • EC 1.11.1.9
  • EGLP
  • epididymal androgen-related protein
  • epididymal secretory glutathione peroxidase
  • Epididymis-specific glutathione peroxidase-like protein
  • glutathione peroxidase 5 (epididymal androgen-related protein)
  • Glutathione peroxidase 5
  • GPx-5
  • GSHPx-5

Background

Glutathione peroxidase (GPX)/glutathione reductase is part of the hydrogen peroxide scavenging system found within the epididymis in the mammalian male reproductive tract. GPX5 expression is epididymis-specific and the transcript is unique from other GPXs because it contains a deletion resulting in an mRNA that does not contain a selenocysteine (UGA) codon (an unusual amino acid present in other GPXs). This deletion also renders the mRNA incapable of encoding an active GPX isoenzyme. For this reason, GPX5 is selenium-independent and has very little activity towards hydrogen peroxide or organic hydroperoxides. GPX5, which is bound to the acrosome of sperm, may act to protect sperm from premature acrosome reaction in the epididymis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GPX5