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Gelsolin/GSN Antibody (20) - BSA Free

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Western Blot: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human cell line U-251 MG shows positivity in actin filaments. Antibody dilution: 1:2000
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human Kidney.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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Gelsolin/GSN Antibody (20) - BSA Free Summary

Immunogen
Gelsolin purified from human plasma
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GSN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:4000
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Immunohistochemistry 1:6000
  • Immunohistochemistry-Paraffin 1:6000
  • Western Blot 1:100-1:2000
Application Notes
ELISA: NBP1-05161 does not work as a capture antibody in sandwich ELISA. WB: NBP1-05161 can be used in Western blotting. IHC: NBP1-05161 can be used in Immunohistochemistry. IF: NBP1-05161 can be used in Immunofluorescence.
Publications
Read Publication using NBP1-05161.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Gelsolin/GSN Antibody (20) - BSA Free

  • Actin-depolymerizing factor
  • ADF
  • AGEL
  • Brevin
  • DKFZp313L0718
  • gelsolin (amyloidosis, Finnish type)
  • Gelsolin
  • GSN

Background

Gelsolin participates in the extracellular actin scavenger system, binding actin and disaggregating filamentous actin released from dying cells. Actin monomers are then transferred to Gc-globulin. The plasma concentration of gelsolin is reduced in conditions of major actin release, such as acute liver failure, major trauma and rhabdomyolysis, where multi-organ failure is a risk. It has been suggested that gelsolin, like Gc-globulin, can be a prognostic factor in these conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Gelsolin/GSN Antibody (NBP1-05161)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Gelsolin/GSN Antibody (NBP1-05161) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSN