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GADD153/CHOP Antibody (9C8) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IP, ChIP, KD
Clone
9C8
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

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GADD153/CHOP Antibody (9C8) [DyLight 405] Summary

Immunogen
Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
Epitope
The N-terminal region of CHOP/GADD153.
Localization
Nuclear
Marker
ER Stress Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDIT3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32828953). Human, mouse, rat and primate.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for GADD153/CHOP Antibody (9C8) [DyLight 405]

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DDIT3