Frizzled-10 Antibody (2678A) [Alexa Fluor® 350] Summary
Additional Information |
Recombinant Monoclonal Antibody. |
Immunogen |
Mouse myeloma cell line NS0-derived human Frizzled-10 Ile21-Gly161 Accession # Q6NSL8 |
Specificity |
Detects human Frizzled-10 in direct ELISAs. |
Source |
N/A |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Purity Statement |
Protein A or G purified from cell culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Protect from light. Do not freeze.- 12 months from date of receipt, 2 to 8 °C as supplied.
|
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Frizzled-10 Antibody (2678A) [Alexa Fluor® 350]
Background
Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the Frizzled family of Wnt receptors (1, 2). The 205 amino acid N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3‑5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). PDZ motifs mediate intracellular binding to scaffolding proteins. Within the cysteine-rich domain, human Frizzled‑10 shares 71% amino acid (aa) sequence identity with Frizzled-9 and 31%-46% with Frizzled-1, -2, -3, -4, -5, - 6, -7, and -8. It shares 96%, 94%, 90%, and 82% aa sequence identity with chick, mouse, Xenopus, and zebrafish Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6-11). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (12, 13). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 to transduce Wnt-7a and Wnt-7b signals, resulting in the stabilization of cytoplasmic beta-catenin (11, 13). Frizzled-10 is also up-regulated in some cancers and transformed cell lines (4, 14). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (15).
- Kikuchi, A. et al. (2007) Cell. Signal. 19:659.
- Wang, H. et al. (2006) Cell. Signal. 18:934.
- Koike, J. et al. (1999) Biochem. Biophys. Res. Commun. 262:39.
- Nagayama, S. et al. (2005) Oncogene 24:6201.
- Dann, C.E. et al. (2001) Nature 412:86.
- Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
- Kawakami, Y. et al. (2000) Mech. Dev. 91:375.
- Nasevicius, A. et al. (2000) Mech. Dev. 92:311.
- Moriwaki, J. et al. (2000) Biochem. Biophys. Res. Commun. 278:377.
- Stark, M.R. et al. (2000) Mech. Dev. 93:195.
- Wang, Z. et al. (2005) Mol. Cell. Biol. 25:5022.
- Nunnally, A.P. and B.A. Parr (2004) Dev. Genes Evol. 214:144.
- Kawakami, Y. et al. (2000) Dev. Growth Differ. 42:561.
- Saitoh, T. et al. (2002) Int. J. Oncol. 20:117.
- Togashi, A. et al. (2005) Cancer Res. 65:4817.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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