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FoxP3 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, WB, Dual ISH-IHC, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-39002AF350
Alexa Fluor 405
NB100-39002AF405
Alexa Fluor 488
NB100-39002AF488
Alexa Fluor 532
NB100-39002AF532
Alexa Fluor 594
NB100-39002AF594
Alexa Fluor 647
NB100-39002AF647
Alexa Fluor 700
NB100-39002AF700
Alexa Fluor 750
NB100-39002AF750
Allophycocyanin
NB100-39002APC
Biotin
NB100-39002B
CoraFluor 1
NB100-39002CL1
DyLight 350
NB100-39002UV
DyLight 405
NB100-39002V
DyLight 488
NB100-39002G
DyLight 550
NB100-39002R
DyLight 594
NB100-39002DL594
DyLight 680
NB100-39002FR
DyLight 755
NB100-39002IR
FITC
NB100-39002F
HRP
NB100-39002H
Janelia Fluor 525
NB100-39002JF525
Janelia Fluor 549
NB100-39002JF549
Janelia Fluor 585
NB100-39002JF585
Janelia Fluor 635
NB100-39002JF635
Janelia Fluor 646
NB100-39002JF646
Janelia Fluor 669
NB100-39002JF669
PE
NB100-39002PE
PerCP
NB100-39002PCP
Unconjugated
NB100-39002
mFluor Violet 450 SE
NB100-39002MFV450
mFluor Violet 500 SE
NB100-39002MFV500
mFluor Violet 610 SE
NB100-39002MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-39002
BSA Free
NB100-39002
BSA Free
NB100-39002SS
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FoxP3 Antibody [DyLight 650] Summary

Immunogen
A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXP3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Single Cell Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771). Bovine reativity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for FoxP3 Antibody [DyLight 650]

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB100-39002C). (Showing 1 - 3 of 3 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

Secondary Antibodies

 

Isotype Controls

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Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.
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Bioinformatics

Gene Symbol FOXP3