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FLAD1 Antibody

Images

 
Western Blot: FLAD1 Antibody [NBP1-84752] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane ...read more
Immunocytochemistry/ Immunofluorescence: FLAD1 Antibody [NBP1-84752] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: FLAD1 Antibody [NBP1-84752] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FLAD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLAD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FLAD1 Protein (NBP1-84752PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FLAD1 Antibody

  • EC 2.7.7
  • FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
  • FAD1
  • Fad1, flavin adenine dinucleotide synthetase, homolog (yeast)
  • FAD-synthetase
  • Flavin adenine dinucleotide synthase
  • flavin adenine dinucleotide synthetase
  • flavin adenine dinucleotide synthetase, homolog
  • MGC31803
  • MGC40255
  • PP591

Background

The FLAD1 gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adeninedinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLAD1