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FGFR5/FGFRL1 Antibody

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FGF R5/FGFRL1 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse FGF R5/FGFRL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1899) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
FGF R5/FGFRL1 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse FGF R5/FGFRL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1899) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF1899-SP
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FGFR5/FGFRL1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R5 beta
Ala21-Pro374
Accession # Q91V87
Specificity
Detects mouse FGF R5 beta in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) FGF R1 alpha  (IIIb), recombinant mouse (rm) FGF R2 beta  (IIIc), rmFGF R3 alpha  (IIIb), rhFGF R3 alpha  (IIIc), rhFGF R1 beta  (IIIc), rmFGF R2 beta  (IIIb), rhFGF R1 alpha  (IIIc), rmFGF R2 alpha  (IIIc), rhFGF R1 beta  (IIIb), rmFGF R2 alpha (IIIb), rmFGF R2, and rmFGF R4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Fgfrl1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1899 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR5/FGFRL1 Antibody

  • FGF homologous factor receptor
  • FGF R5
  • FGF receptor-like protein 1
  • FGFR5
  • FGFR-5
  • FGFRL1
  • FGFR-like protein
  • FHFR
  • Fibroblast growth factor receptor 5
  • fibroblast growth factor receptor-like 1

Background

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2-6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).
  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Sleeman, M. et al. (2001) Gene 271:171.
  3. Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
  4. Antoine, M. et al. (2005) Growth Factors 23:87.
  5. Wiedemann, M. et al. (2000) Genomics 69:275.
  6. Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FGFR5/FGFRL1 Antibody
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Applications: WB, IHC

Publications for FGFR5/FGFRL1 Antibody (AF1899)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-Fr, IHC-P.


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(1)
IHC-P
(1)
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(2)
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Bioinformatics

Gene Symbol Fgfrl1
Uniprot