Home » FGFR1-4 » FGFR1-4 Antibodies » FGFR1-4 [p Tyr653, p Tyr654, p Tyr654] Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

FGFR1-4 [p Tyr653, p Tyr654, p Tyr654] Antibody [Unconjugated]

 (8 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 ...read more
FGF R1-4 phosphorylated at Y653/Y654 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-FGF R1-4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3285-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

FGFR1-4 [p Tyr653, p Tyr654, p Tyr654] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human FGF R1 Y653/Y654 sites
Modification
p Tyr654, p Tyr653, p Tyr654
Specificity
Detects human FGF R when dually phosphorylated at Y653/Y654 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3285 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR1-4 [p Tyr653, p Tyr654, p Tyr654] Antibody [Unconjugated]

  • BFGFR;bFGF-R-1;CD331;CEK;ECCL;FGFBR;FGFR-1;Fibroblast growth factor receptor;Fibroblast growth factor receptor 1;FLG;FLT2;FLT-2;HBGFR;HH2;HRTFDS;KAL2;N-SAM;OGD
  • FGFR1-4

Background

The fibroblast growth factor receptors (FGF R), including FGF R1, R2, R3, and R4, are involved in development processes, angiogenesis, wound healing, and tumorigenesis. FGF binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Tyr653 and Tyr654 in FGF R1 and the corresponding tyrosine residues in FGF R2, R3 and R4 are important for the activation of intrinsic kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(547 Publications)
Species: Hu
Applications: BA
FGFR2 Antibody
(1G3)
H00002263-M01
Immunohistochemistry-Paraffin FGFR2 Antibody (1G3)
Western Blot FGFR2 Antibody (1G3)
Sandwich ELISA FGFR2 Antibody (1G3)
(13 Publications)
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
FGF acidic/FGF1
Antibody [Unconjugate
AF232
Immunohistochemistry FGF acidic/FGF1 Antibody [Unconjugated]
Simple Western FGF acidic/FGF1 Antibody [Unconjugated]
Western Blot FGF acidic/FGF1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: IHC, Neut, Simple Western, WB
FGFR3 Antibody
(136312) [Unconjugate ...
MAB7662
Immunohistochemistry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
Flow Cytometry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
Immunocytochemistry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
(1 Review)
(3 Publications)
Species: Hu
Applications: Flow, ICC, IHC, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
FGFR4 Antibody
(240929) [Unconjugate
MAB6852
Immunocytochemistry FGFR4 Antibody (240929) [Unconjugated]
Immunohistochemistry FGFR4 Antibody (240929) [Unconjugated]
Western Blot FGFR4 Antibody (240929) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(682 Publications)
Species: Hu
Applications: ELISA
FGF-8
[Unconjugated]
423-F8
SDS-PAGE FGF-8 [Unconjugated]
Bioactivity FGF-8 [Unconjugated]
Cell Culture FGF-8 [Unconjugated]
(1 Review)
(62 Publications)
Species: Hu, Mu
Applications: BA
FGF-13 Antibody
NBP2-16471
Immunohistochemistry-Paraffin FGF-13 Antibody
Western Blot FGF-13 Antibody
Immunohistochemistry-Paraffin FGF-13 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
KGF/FGF-7
[Unconjugated]
251-KG
Bioactivity KGF/FGF-7 [Unconjugated]
Bioactivity KGF/FGF-7 [Unconjugated]
(85 Publications)
Species: Hu
Applications: BA
KAL1 Antibody -
Azide and BSA Free
NBP3-03937
Western Blot KAL1 Antibody - Azide and BSA Free
Immunohistochemistry KAL1 Antibody - Azide and BSA Free
Immunohistochemistry KAL1 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
Carbonic Anhydrase
I/CA1 Antibody
NBP1-88191
Western Blot Carbonic Anhydrase I/CA1 Antibody
Immunohistochemistry-Paraffin Carbonic Anhydrase I/CA1 Antibody
Immunohistochemistry-Paraffin Carbonic Anhydrase I/CA1 Antibody
(3 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(62 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
PDGF R beta
Antibody [Unconjugate
AF1042
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
Western Blot PDGF R beta Antibody [Unconjugated]
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
(107 Publications)
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
FGF-10
[Unconjugated]
345-FG
SDS-PAGE FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
(103 Publications)
Species: Hu
Applications: BA

Publications for FGFR1-4 Antibody (AF3285)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, ICC/IF, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
ICC/IF
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF3285 Applications Species
T Shirakihar, H Yamaguchi, T Kondo, M Yashiro, R Sakai Transferrin receptor 1 promotes the fibroblast growth factor receptor-mediated oncogenic potential of diffused-type gastric cancer Oncogene, 2022-03-25;0(0):. 2022-03-25 [PMID: 35338344] (ICC/IF, Western Blot, Human) ICC/IF, Western Blot Human
Hong Xiang, Abigael G. Chan, Ago Ahene, David I. Bellovin, Rong Deng, Amy W. Hsu et al. Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer mAbs 11/1/2021 [PMID: 34719330]
Kyeong Won Lee, Jae‐Yeon Jeong, Young Jun An, Jung‐Hyun Lee, Hyung‐Soon Yim FGF11 influences 3T3‐L1 preadipocyte differentiation by modulating the expression of PPAR gamma regulators FEBS Open Bio 4/1/2019 [PMID: 30984550]
Y Cha, HP Kim, Y Lim, SW Han, SH Song, TY Kim FGFR2 amplification is predictive of sensitivity to regorafenib in gastric and colorectal cancers in vitro Mol Oncol, 2018-05-29;0(0):. 2018-05-29 [PMID: 29573334] (Western Blot, Human) Western Blot Human
L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth Cell Death Dis, 2018-02-15;9(3):262. 2018-02-15 [PMID: 29449529] (Western Blot, Human) Western Blot Human
D Nawrocka, K Kornicka, J Szydlarska, K Marycz Basic Fibroblast Growth Factor Inhibits Apoptosis and Promotes Proliferation of Adipose-Derived Mesenchymal Stromal Cells Isolated from Patients with Type 2 Diabetes by Reducing Cellular Oxidative Stress Oxid Med Cell Longev, 2017-01-11;2017(0):3027109. 2017-01-11 [PMID: 28168007] (ICC, Human) ICC Human
KAI LIU, XILIN SONG, MEIRONG ZHU, HENG MA Overexpression of FGFR2 contributes to inherent resistance to MET inhibitors in MET-amplified patient-derived gastric cancer xenografts Oncology Letters 10/1/2015 [PMID: 26622787]
Kunii K, Davis L, Gorenstein J, Hatch H, Yashiro M, Di Bacco A, Elbi C, Lutterbach B FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival. Cancer Res., 2008-04-01;68(7):2340-8. 2008-04-01 [PMID: 18381441] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human

Reviews for FGFR1-4 Antibody (AF3285) (0)

There are no reviews for FGFR1-4 Antibody (AF3285). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGFR1-4 Antibody (AF3285) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional FGFR1-4 Products

Array AF3285

Blogs on FGFR1-4

There are no specific blogs for FGFR1-4, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FGFR1-4 [p Tyr653, p Tyr654, p Tyr654] Antibody [Unconjugated] and receive a gift card or discount.