Home » FALDH » FALDH Lysates » FALDH Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

FALDH Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: FALDH Overexpression Lysate (Adult Normal) [NBP2-11169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FALDH.
Western Blot: FALDH Overexpression Lysate (Adult Normal) [NBP2-11169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FALDH.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

FALDH Overexpression Lysate Summary

Description

FALDH Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC223398

Accession#: NM_000382

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
ALDH3A2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
54.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for FALDH Overexpression Lysate

  • Aldehyde dehydrogenase 10
  • aldehyde dehydrogenase 3 family, member A2
  • Aldehyde dehydrogenase family 3 member A2
  • ALDH10FLJ20851
  • EC 1.2.1
  • EC 1.2.1.3
  • FALDHDKFZp686E23276
  • fatty aldehyde dehydrogenase
  • Microsomal aldehyde dehydrogenase
  • SLS

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Mutations in the gene cause Sjogren-Larsson syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

LIGHT/TNFSF14
[Unconjugated]
664-LI
SDS-PAGE LIGHT/TNFSF14 [Unconjugated]
Bioactivity LIGHT/TNFSF14 [Unconjugated]
(21 Publications)
Species: Hu
Applications: BA
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
Desmocollin-3
Antibody (765129)
MAB7265
Western Blot Desmocollin-3 Antibody (765129)
Immunohistochemistry Desmocollin-3 Antibody (765129)
(1 Review)
Species: Mu
Applications: IHC, WB
Aldehyde
Dehydrogenase 3-A1/AL ...
NBP2-02483
Western Blot Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6)
Flow Cytometry Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6)
Western Blot Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
CD19 Antibody
(CB19) - BSA Free
NBP2-25196
Flow Cytometry CD19 Antibody (CB19) - BSA Free
Flow Cytometry CD19 Antibody (CB19) - BSA Free
Flow Cytometry CD19 Antibody (CB19) - BSA Free
(1 Review)
(12 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, In vitro, WB
Afamin Antibody
NBP1-89156
Immunohistochemistry-Paraffin Afamin Antibody
Immunohistochemistry-Paraffin Afamin Antibody
Immunohistochemistry-Paraffin Afamin Antibody
Species: Hu
Applications: IHC, IHC-P
PPAR alpha/NR1C1
Antibody (3B6/PPAR) - ...
NB300-537
Flow Cytometry PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free
Western Blot PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free
Flow Cytometry PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free
(1 Review)
(16 Publications)
Species: Bv, Hu, Mu, Rb, Rt
Applications: ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, KD, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(661 Publications)
Species: Mu
Applications: ELISA
AKR1A1 Antibody
(OTI6E3)
NBP2-02164
Flow Cytometry AKR1A1 Antibody (OTI6E3)
Flow Cytometry AKR1A1 Antibody (OTI6E3)
Flow Cytometry AKR1A1 Antibody (OTI6E3)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
ERMAP Antibody
[Unconjugated]
AF4928
Flow Cytometry ERMAP Antibody [Unconjugated]
Species: Hu
Applications: CyTOF-ready, Flow, WB
Gastrin Antibody
NBP3-12223
Immunohistochemistry-Paraffin Gastrin Antibody
Unknown Gastrin Antibody
Species: Hu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB

N-Acetylgalactosamine- ...
NBP1-32899
Western Blot N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody
Western Blot N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody
Immunocytochemistry/ Immunofluorescence N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody
(2 Reviews)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PA1 Antibody
NBP1-06542
Immunohistochemistry PA1 Antibody
Immunohistochemistry-Paraffin PA1 Antibody
Western Blot PA1 Antibody
Species: Hu
Applications: IHC, IHC-P, IP, WB (-)
PKD2L1 Antibody
(4F9)
H00009033-M01
Western Blot PKD2L1 Antibody (4F9)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, PAGE, WB
VEGFR3/Flt-4
Antibody [Unconjugate
AF743
Flow Cytometry VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
(3 Reviews)
(140 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, WB
USH1G Antibody
NBP1-89076
Immunohistochemistry-Paraffin USH1G Antibody
(1 Publication)
Species: Ca, Hu
Applications: IHC, IHC-P, WB
B7-2/CD86 Antibody
(BU63)
NBP2-25208
Knockout Validated B7-2/CD86 Antibody (BU63)
Immunocytochemistry/ Immunofluorescence B7-2/CD86 Antibody (BU63)
Flow Cytometry B7-2/CD86 Antibody (BU63)
(27 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
FALDH
Overexpression Lysate
NBP2-11169
FALDH Overexpression Lysate
Species: Hu
Applications: WB

Publications for FALDH Lysate (NBP2-11169) (0)

There are no publications for FALDH Lysate (NBP2-11169).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FALDH Lysate (NBP2-11169) (0)

There are no reviews for FALDH Lysate (NBP2-11169). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FALDH Lysate (NBP2-11169) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FALDH Products

Research Areas for FALDH Lysate (NBP2-11169)

Find related products by research area.

Blogs on FALDH

There are no specific blogs for FALDH, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FALDH Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol ALDH3A2