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ETV3 Antibody

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Western Blot: ETV3 Antibody [NBP1-80931] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4
Western Blot: ETV3 Antibody [NBP1-80931] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: ETV3 Antibody [NBP1-80931] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ETV3 Antibody [NBP1-80931] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ETV3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EKEEHTQEEGTVPSRTIEEEKGTIFARPAAPPIWPSVPISTPSGEPLEVTEDSEDRPGKEPSAPEKKEDALMPPKLRLKRRWNDDPEARELSKSGKFLWNG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETV3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ETV3 Protein (NBP1-80931PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ETV3 Antibody

  • bA110J1.4
  • ETS domain transcriptional repressor PE1
  • ETS translocation variant 3
  • ets variant 3
  • ets variant gene 3, ETS family transcriptional repressor
  • METS
  • Mitogenic Ets transcriptional suppressor
  • PE-1ets variant gene 3
  • PE1FLJ79173

Background

The ETV3 gene encodes an ETS translocation variant 3 protein, that in isoform 1 is 512 amino acids long at 57 kDA and in isoform 2 is 143 amino acids long at nearly 17 kDA. This protein functions in terminal macrophage differentiation as a transcriptional repressor that contributes to growth arrest. It also represses MMP1 promoter activity and interacts with the MYC and DDX20 genes. ETV3 is linked to diseases heart disease, hypertension, ADHD, and conduct disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ETV3
Entrez