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ErbB4/Her4 Antibody (5G6B4) - BSA Free

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Western Blot: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis using ErbB4/Her4 mAb against human ERBB4 (AA: 1159-1308) recombinant protein. (Expected MW is 43.3 kDa).
Western Blot: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis using ErbB4/Her4 mAb against human ERBB4 (AA: 1159-1308) recombinant protein. (Expected MW is 43.3 kDa).
Immunohistochemistry-Paraffin: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis of paraffin-embedded cervical cancer tissues using ERBB4 mouse mAb with DAB staining.
Flow Cytometry: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis of Hela cells using ERBB4 mouse mAb (green) and negative control (red).
Western Blot: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis using ERBB4 mAb against HEK293 (1) and ERBB4 -hIgGFc transfected HEK293 (2) cell lysate.
ELISA: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
5G6B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52462-0.025mg
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ErbB4/Her4 Antibody (5G6B4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human ErbB4/Her4 (AA: 1159-1308) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ERBB4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
146.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for ErbB4/Her4 Antibody (5G6B4) - BSA Free

  • avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB4
  • HER4
  • HER4MGC138404
  • p180erbB4
  • Proto-oncogene-like protein c-ErbB-4
  • receptor tyrosine-protein kinase erbB-4
  • Tyrosine kinase-type cell surface receptor HER4
  • v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Background

erbB4/HER4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. erbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). erbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ErbB4/Her4
Antibody (5G6B4) - BS
NBP2-52462
ErbB4/Her4 Antibody (5G6B4) - BSA Free
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ERBB4