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EphB3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] - Staining in human skin and skeletal muscle tissues using anti-EPHB3 antibody. Corresponding EPHB3 RNA-seq data are presented for ...read more
Western Blot: EphB3 Antibody [NBP1-84776] - Analysis in control (vector only transfected HEK293T lysate) and EPHB3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] - Staining of human skin shows high expression.
Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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EphB3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVP
Predicted Species
Mouse (95%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPHB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
EphB3 Protein (NBP1-84776PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EphB3 Antibody

  • Cek10
  • EC 2.7.10
  • EC 2.7.10.1
  • EK2
  • EPH receptor B3
  • EphB3
  • EPH-like kinase 2
  • EPH-like tyrosine kinase-2
  • ephrin type-B receptor 3
  • ETK2Hek2
  • Hek2
  • human embryo kinase 2
  • Mdk5
  • Sek4
  • Tyro6
  • Tyrosine-protein kinase TYRO6

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EPHB3