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EphB2 Antibody (2D12C6) - BSA Free

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Western Blot: EphB2 Antibody (2D12C6) [NBP1-47525] - Analysis using EphB2 mouse mAb against truncated EphB2 recombinant protein (1) and extracellular EphB2(aa19-476)-hIgGFc transfected CHO-K1 cell lysate(2).
Immunocytochemistry/ Immunofluorescence: EphB2 Antibody (2D12C6) [NBP1-47525] - Analysis of porcine outgrowth endothelial cells isolated from fetal porcine blood cord (pOECs) using EphB2 antibody. Primary antibody ...read more
Immunocytochemistry/ Immunofluorescence: EphB2 Antibody (2D12C6) [NBP1-47525] - Analysis of Hela (left) and HepG2 (right) cells using EphB2 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 ...read more

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2D12C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

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EphB2 Antibody (2D12C6) - BSA Free Summary

Immunogen
Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EPHB2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Reviewed Applications
Read 1 Review rated 4
using
NBP1-47525 in the following applications:

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for EphB2 Antibody (2D12C6) - BSA Free

  • CAPB
  • Cek5
  • Drt
  • DRTEphB2
  • EC 2.7.10
  • EC 2.7.10.1
  • EK5
  • elk-related tyrosine kinase
  • EPH receptor B2
  • eph tyrosine kinase 3
  • EphB2
  • EPH-like kinase 5
  • ephrin type-B receptor 2
  • EPHT3MGC87492
  • EPTH3
  • Erk
  • ERKHek5
  • Hek5
  • Nuk
  • PCBC
  • protein-tyrosine kinase HEK5
  • Qek2
  • Renal carcinoma antigen NY-REN-47
  • Sek3
  • Tyro5
  • Tyrosine-protein kinase receptor EPH-3
  • Tyrosine-protein kinase TYRO5

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB2 Antibody (NBP1-47525) (0)

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Review for EphB2 Antibody (NBP1-47525) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Porcine.

Reviews using NBP1-47525:
Filter by Applications
Immunofluorence in 2D culture
(1)
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Porcine
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Sandra Meliton Barbancho
Immunofluorence in 2D culture Porcine 12/02/2022
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Summary

ApplicationOther
Sample Testedporcine endothelial cells
SpeciesPorcine
Lot160728

Comments

CommentsPorcine endothelial cells isolated from fetal blood cord
***Novus Innovators Program - new species or application used on a primary antibody.***

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EphB2 Antibody (NBP1-47525) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional EphB2 Products

Research Areas for EphB2 Antibody (NBP1-47525)

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Blogs on EphB2.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Sandra Meliton Barbancho
12/02/2022
Application: Immunofluorence in 2D culture
Species: Porcine

Bioinformatics

Gene Symbol EPHB2
Entrez