![Recombinant Human DEPTOR/DEPDC6 Protein [H00064798-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-DEPTOR-DEPDC6-Protein-H00064798-P01-img0001.jpg "Recombinant Human DEPTOR/DEPDC6 Protein [H00064798-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Func, IHC, MA, AP |
| Description | A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-409 of Human DEPDC6 Source: Wheat Germ (in vitro) Amino Acid Sequence: MEEGGSTGSAGSDSSTSGSGGAQQRELERMAEVLVTGEQLRLRLHEEKVIKDRRHHLKTYPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSSKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKTVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVNNLILTGPRTIVMEVMEELEC |
| Preparation Method |
in vitro wheat germ expression system |
| Details of Functionality | This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated. |
| Source | Wheat germ |
| Protein/Peptide Type | Recombinant Protein |
| Gene | DEPTOR |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
| Dilutions |
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| Application Notes | Use in In vitro assay reported in scientific literature (PMID 26795633). Use in IHC and WB reported in scientific literature (PMID 31395691). |
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| Theoretical MW | 70.73 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer. |
| Preservative | No Preservative |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
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Research Areas for DEPTOR/DEPDC6 Recombinant Protein (H00064798-P01)Find related products by research area.
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| Gene Symbol | DEPTOR |
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![Knockout Validated: DEPTOR/DEPDC6 Antibody - BSA Free [NBP1-49674] - Whole-body DEPTOR null mice are viable, fertile, normal in size, and do not display any gross physical abnormalities. Western blot showing the expression of DEPTOR/DEPDC6 in tissues of Deptor wild-type (wt/wt), heterozygote (Delta/wt) or knockout (Delta/Delta) mice. Akt was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28462079/) licensed under a CC-BY license.](https://images.novusbio.com/images/DEPTOR-DEPDC6 Antibody - BSA Free-Knockout Validated-NBP1-49674-img0013.jpg "Knockout Validated: DEPTOR/DEPDC6 Antibody - BSA Free [NBP1-49674] - Whole-body DEPTOR null mice are viable, fertile, normal in size, and do not display any gross physical abnormalities. Western blot showing the expression of DEPTOR/DEPDC6 in tissues of Deptor wild-type (wt/wt), heterozygote (Delta/wt) or knockout (Delta/Delta) mice. Akt was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28462079/) licensed under a CC-BY license.")