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DENND1B Overexpression Lysate

Name: DENND1B Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-05522
Price: 679 USD

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Western Blot: DENND1B Overexpression Lysate (Adult Normal) [NBP2-05522] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DENND1B.

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB

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DENND1B Overexpression Lysate Summary

Description

DENND1B Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC205585

Accession#: NM_144977

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Gene

DENND1B

Applications/Dilutions

Dilutions	Western Blot
Application Notes	This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW	44.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	RIPA buffer

Lysate Details for Array

Type

Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for DENND1B Overexpression Lysate

C1ORF18
C1orf218
chromosome 1 open reading frame 218
Connecdenn 2
DENN domain-containing protein 1B
DENN/MADD domain containing 1B
DKFZp547O0715
FAM31BMGC103810
FLJ20054
FLJ21179
FLJ33888
member B
Protein FAM31B

Background

DENND1B is a member of the connecdenn family, which links RAB35 activation with the clathrin machinery. The protein functions as a guanine nucleotide exchange factor for RAB35. DENND1B is present at higher levels in dendritic and natural killer cells but at lower levels in the other myeloid lineage cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol

DENND1B

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

DENND1B Overexpression Lysate

Species Glossary

Images

Product Details

Order Details

DENND1B Overexpression Lysate Summary

DENND1B Transient Overexpression Lysate

Applications/Dilutions

Packaging, Storage & Formulations

Lysate Details for Array

Notes

Alternate Names for DENND1B Overexpression Lysate

Background

Limitations

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Publications for DENND1B Lysate (NBP2-05522) (0)

Reviews for DENND1B Lysate (NBP2-05522) (0)

Product General Protocols

Video Protocols

FAQs for DENND1B Lysate (NBP2-05522) (0)

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Blogs on DENND1B

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Bioinformatics