![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DDX21 Antibody NB100-1718 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0015.jpg "Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DDX21 Antibody NB100-1718 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
| Reactivity | Hu, Mu, Ze, Rt, Po, Ca, Eq, Pm, RM, SqSpecies Glossary |
| Applications | WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 725 and the C-terminus (residue 783) of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188). |
| Predicted Species | Rat (100%), Porcine (100%), Squirrel (100%), Rhesus Macaque (100%), Canine (100%), Primate (100%), Equine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DDX21 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 87 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Yuwei Li |
WB | Human | 03/13/2024 |
Summary
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Secondary Antibodies |
Isotype Controls |
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The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura. It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol... Read full blog post. |
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![Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on Jurkat Cells with DDX21Antibody NB100-1718 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).](http://images.novusbio.com/fullsize/DDX21-Antibody-Flow-Cytometry-NB100-1718-img0011.jpg "Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on Jurkat Cells with DDX21Antibody NB100-1718 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).")
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with DDX21 Antibody conjugated to Alexa Fluor 488 (NB100-1718AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0016.jpg "Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with DDX21 Antibody conjugated to Alexa Fluor 488 (NB100-1718AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - ICC/IF detection of DDX21 in HeLa cells which were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-DDX21 [NB100-1718] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DylightTM 488 (green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DylightTM 550 (red) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue) [NBP2-31156].](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0010.jpg "Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - ICC/IF detection of DDX21 in HeLa cells which were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-DDX21 [NB100-1718] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DylightTM 488 (green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DylightTM 550 (red) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue) [NBP2-31156].")
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to Alexa Fluor 647 (NB100-1718AF647) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0017.jpg "Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to Alexa Fluor 647 (NB100-1718AF647) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-DDX21 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0013.jpg "Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-DDX21 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-DDX21 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0014.jpg "Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-DDX21 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: DDX21 Antibody [NB100-1718] - Detection of Human DDX21 on HeLa whole cell lystate using NB100-1718. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1716 and NB100-1717.](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NB100-1718-img0004.jpg "Western Blot: DDX21 Antibody [NB100-1718] - Detection of Human DDX21 on HeLa whole cell lystate using NB100-1718. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1716 and NB100-1717.")
![Immunohistochemistry: DDX21 Antibody [NB100-1718] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX21 used at a dilution of 1:200 (1ug/ml) . Detection: DAB.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-NB100-1718-img0007.jpg "Immunohistochemistry: DDX21 Antibody [NB100-1718] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX21 used at a dilution of 1:200 (1ug/ml) . Detection: DAB.")
![Flow Cytometry: DDX21 Antibody - BSA Free [NB100-1718] - An intracellular stain was performed on A431 cells with DDX21 NB100-1718AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/DDX21-Antibody---BSA-Free-Flow-Cytometry-NB100-1718-img0019.jpg "Flow Cytometry: DDX21 Antibody - BSA Free [NB100-1718] - An intracellular stain was performed on A431 cells with DDX21 NB100-1718AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of human larynx squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NB100-1718-img0005.jpg "Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of human larynx squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.")
![Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NB100-1718-img0006.jpg "Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.")
![Immunohistochemistry: DDX21 Antibody [NB100-1718] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX21 used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-NB100-1718-img0008.jpg "Immunohistochemistry: DDX21 Antibody [NB100-1718] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX21 used at a dilution of 1:200 (1ug/ml). Detection: DAB.")
![Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on HeLa cells with DDX21 Antibody NB100-1718AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/DDX21-Antibody-Flow-Cytometry-NB100-1718-img0018.jpg "Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on HeLa cells with DDX21 Antibody NB100-1718AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
