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DARPP-32 Antibody (2A4G9) - BSA Free

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Western Blot: DARPP-32 Antibody (2A4G9) [NBP2-37302] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: DARPP-32 Antibody (2A4G9) [NBP2-37302] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: DARPP-32 Antibody (2A4G9) [NBP2-37302] - Western blot analysis using PPP1R1B mAb against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)
ELISA: DARPP-32 Antibody (2A4G9) [NBP2-37302] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2A4G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37302-0.025ml
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DARPP-32 Antibody (2A4G9) - BSA Free Summary

Immunogen
Purified recombinant fragment of human DARPP-32 (AA: 95-204) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPP1R1B
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for DARPP-32 Antibody (2A4G9) - BSA Free

  • DARPP32
  • DARPP-32
  • PPP1R1B
  • protein phosphatase 1, regulatory (inhibitor) subunit 1B
  • regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DARPP-32 Antibody
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NBP2-37302
DARPP-32 Antibody (2A4G9) - BSA Free
Species: Hu
Applications: WB, ELISA

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Bioinformatics

Gene Symbol PPP1R1B