![Western Blot: Cystatin-8 Antibody [NBP1-47219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 with C-terminal MYC tag probed with (0.3ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cystatin-8-Antibody-Western-Blot-NBP1-47219-img0002.jpg "Western Blot: Cystatin-8 Antibody [NBP1-47219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 with C-terminal MYC tag probed with (0.3ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-DPKKNETGVLRK corresponding to internal region according to NP_005483.1. |
| Epitope | Internal region |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CST8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: In transfected HEK293 transiently expressing human CST8 (myc and DYKDDDDK tagged), a band of approx. 23 kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-47219 | Applications | Species |
|---|---|---|
| Chapuis J, Hot D, Hansmannel F et al. Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease. Mol Psychiatry 2009-11-01 [PMID: 19204726] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cystatin-8 Antibody (NBP1-47219)Find related products by research area.
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| Gene Symbol | CST8 |
| Entrez |
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| Uniprot |
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