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CXCL12/SDF-1 Antibody [Unconjugated]

 (24 Publications)
 (2 Reviews)
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Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-HumanCXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND50 is typically 1.5-6.0 µg/mL.
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF310
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-310-SP
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CXCL12/SDF-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL12
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 1.5-6.0 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF-310-NA in the following applications:
Publications
Read Publications using
AF-310-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL12/SDF-1 Antibody [Unconjugated]

  • chemokine (C-X-C motif) ligand 12
  • C-X-C motif chemokine 12
  • CXCL12
  • hIRH
  • hSDF-1
  • Intercrine reduced in hepatomas
  • IRH
  • PBSF
  • PBSFSDF-1a
  • Pre-B cell growth-stimulating factor
  • SCYB12
  • SDF1
  • SDF-1
  • SDF1A
  • SDF1B
  • SDF1SDF-1b
  • stromal cell-derived factor 1
  • TLSF
  • TLSF-a
  • TLSF-b
  • TPAR1

Background

CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL12/SDF-1 Antibody (AF-310-NA)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Blocking/Neutralizing, ELISA Development (Capture), Functional Assay, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
Blocking/Neutralizing
(1)
ELISA Development (Capture)
(1)
Functional Assay
(3)
ICC
(3)
IHC
(1)
IHC-P
(1)
Neutralization
(6)
Western Blot
(2)
All Applications
Filter By Species
Human
(15)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF-310-NA Applications Species
Amber N. Stratman, Margaret C. Burns, Olivia M. Farrelly, Andrew E. Davis, Wenling Li, Van N. Pham et al. Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment Communications Biology 12/4/2020 [PMID: 33277595]
Charlotte T. Rodrigues Neves, Sander W. Spiekstra, Niels P. J. de Graaf, Thomas Rustemeyer, Albert J. Feilzer, Cees J. Kleverlaan et al. Titanium salts tested in reconstructed human skin with integrated MUTZ ‐3‐derived Langerhans cells show an irritant rather than a sensitizing potential Contact Dermatitis 11/1/2020 [PMID: 32677096]
V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin Platelets Fuel the Inflammasome Activation of Innate Immune Cells Cell Rep, 2020-05-12;31(6):107615. 2020-05-12 [PMID: 32402278] (Blocking/Neutralizing, Human) Blocking/Neutralizing Human
Miao He, Weiwei Yu, Chawnshang Chang, Hiroshi Miyamoto, Xiaohong Liu, Ke Jiang et al. Estrogen receptor alpha promotes lung cancer cell invasion via increase of and cross‐talk with infiltrated macrophages through the CCL2/CCR2/MMP9 and CXCL12/CXCR4 signaling pathways Molecular Oncology 8/1/2020 [PMID: 32356397]
JL Viñas, M Spence, A Gutsol, W Knoll, D Burger, J Zimpelmann, DS Allan, KD Burns Receptor-Ligand Interaction Mediates Targeting of Endothelial Colony Forming Cell-derived Exosomes to the Kidney after Ischemic Injury Sci Rep, 2018-11-05;8(1):16320. 2018-11-05 [PMID: 30397255] (Neutralization, Human) Neutralization Human
Lu Yu, Liangli Yu, Quynhchi Pham, Thomas T. Y. Wang Transcriptional and translational‐uncoupling in regulation of the CXCL12 and its receptors CXCR4, 7 in THP‐1 monocytes and macrophages Immunity, Inflammation and Disease 3/1/2018 [PMID: 29105376]
Nancy Adriana Espinoza-Sánchez, Eduardo Vadillo, Juan Carlos Balandrán, Alberto Monroy-García, Rosana Pelayo, Ezequiel M. Fuentes-Pananá Evidence of lateral transmission of aggressive features between different types of breast cancer cells International Journal of Oncology 11/1/2017 [PMID: 29048610]
Harsh Samarendra, Keaton Jones, Tatjana Petrinic, Michael A Silva, Srikanth Reddy, Zahir Soonawalla et al. A meta-analysis of CXCL12 expression for cancer prognosis British Journal of Cancer 6/27/2017 [PMID: 28535157]
Ilona J Kosten, Sander W Spiekstra, Tanja D de Gruijl, Susan Gibbs MUTZ-3 Langerhans Cell maturation and CXCL12 independent migration in reconstructed human gingiva ALTEX 1/1/2016 [PMID: 27196738]
Hanes M, Salanga C, Chowdry A, Comerford I, McColl S, Kufareva I, Handel T Dual targeting of the chemokine receptors CXCR4 and ACKR3 with novel engineered chemokines. J Biol Chem, 2015-07-27;290(37):22385-97. 2015-07-27 [PMID: 26216880] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Show All 24 Publications.

Reviews for CXCL12/SDF-1 Antibody (AF-310-NA) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using AF-310-NA:
Filter by Applications
IP
(2)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IP Human 01/07/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 23009808
SpeciesHuman
  4
reviewed by:
Verified Customer
 IP Other 01/07/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 23009808
SpeciesOther

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Recent Reviews

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 Verified Customer
01/07/2015
Application: IP
Species: Human
read this review
 
 Verified Customer
01/07/2015
Application: IP
Species: Other
read this review

Bioinformatics

Gene Symbol CXCL12
Uniprot