Home » CX3CL1/Fractalkine » CX3CL1/Fractalkine Antibodies » CX3CL1/Fractalkine Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CX3CL1/Fractalkine Antibody [Unconjugated]

 (17 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Rat CX3CL1/Fractalkine (Catalog # 536-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CX3CL1/Fractalkine (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537). The ND50 is typically 0.3-1.2 µg/mL.
Recombinant Rat CX3CL1/Fractalkine (Catalog # 536-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
CX3CL1/Fractalkine was detected in perfusion fixed frozen sections of mouse brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF537
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF537-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CX3CL1/Fractalkine Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat CX3CL1/Fractalkine
Gln25-Gly100
Accession # O55145
Specificity
Detects rat CX3CL1/Fractalkine in ELISAs and Western blots. Neutralizes the biological activity of recombinant human CX3CL1 and recombinant mouse CX3CL1 with similar effectiveness.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CX3CL1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.3-1.2 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Rat CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # AF537)
ELISA Detection:Rat CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog # BAF537)
Standard: Recombinant Rat CX3CL1/Fractalkine (Catalog # 536-FR)
Publications
Read Publications using
AF537 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody [Unconjugated]

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 74 aa or 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino acid sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity.

  1. Kledal, T.N. et al. (1998) FEBS Lett. 441:209.
  2. Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.
  3. Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.
  4. Rossi, D.L. et al. (1998) Genomics 47:163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CX3CR1 Antibody
[Unconjugated]
AF5825
Flow Cytometry CX3CR1 Antibody [Unconjugated]
Western Blot CX3CR1 Antibody [Unconjugated]
(1 Review)
(16 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(255 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
Neurturin
1297-NE/CF
Neurturin
(9 Publications)
Species: Hu
Applications: Bind, BA
GDNF [Unconjugated]
212-GD
Bioactivity GDNF [Unconjugated]
SDS-PAGE GDNF [Unconjugated]
SEC-MALS GDNF [Unconjugated]
(217 Publications)
Species: Hu
Applications: Bind, BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(661 Publications)
Species: Mu
Applications: ELISA
CD35 Antibody (E11)
NBP2-29460
Immunohistochemistry-Paraffin CD35 Antibody (E11)
(6 Publications)
Species: Pm, Pm-Cm, Hu, Pm, RM
Applications: Flow, ICC/IF, IHC, IHC-P, ISH
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(155 Publications)
Species: Hu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(452 Publications)
Species: Hu
Applications: BA
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(183 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
CXCL12/SDF-1 alpha
[Unconjugated]
350-NS
SDS-PAGE CXCL12/SDF-1 alpha [Unconjugated]
Bioactivity CXCL12/SDF-1 alpha [Unconjugated]
(188 Publications)
Species: Fe, Hu, RM
Applications: BA, BA
CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA
CCL17/TARC [HRP]
MCC170
N/A CCL17/TARC [HRP]
N/A CCL17/TARC [HRP]
(45 Publications)
Species: Mu
Applications: ELISA
Ret Antibody
[Unconjugated]
AF482
Immunohistochemistry Ret Antibody [Unconjugated]
Western Blot Ret Antibody [Unconjugated]
Immunohistochemistry Ret Antibody [Unconjugated]
(40 Publications)
Species: Mu
Applications: IHC, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(301 Publications)
Species: Mu
Applications: ELISA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CX3CL1/Fractalkine
Antibody [Unconjugate ...
AF537
CX3CL1/Fractalkine Antibody [Unconjugated]
Species: Rt
Applications: WB, IHC, B/N, ELISA(Cap)

Publications for CX3CL1/Fractalkine Antibody (AF537)(17)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ICC, IHC, IHC-P, In Vivo, Neutralization, Western Blot.


Filter By Application
ICC
(3)
IHC
(3)
IHC-P
(1)
In Vivo
(1)
Neutralization
(2)
Western Blot
(3)
All Applications
Filter By Species
Mouse
(1)
Rat
(8)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF537 Applications Species
Sun, P;Lin, W;Weng, Y;Gong, J;Huang, Y;Tang, Y;Lin, C;Chen, A;Chen, Y; Spinal Cathepsin S promotes visceral hypersensitivity via FKN/CX3CR1/p38 MAPK signaling pathways Molecular pain 2023-06-22 [PMID: 37347150] (In Vivo, Rat) In Vivo Rat
Y Shen, J Cui, S Zhang, Y Wang, J Wang, Y Su, D Xu, Y Liu, Y Guo, W Bai Temporal alteration of microglia to microinfarcts in rat brain induced by the vascular occlusion with fluorescent microspheres Frontiers in Cellular Neuroscience, 2022-08-03;16(0):956342. 2022-08-03 [PMID: 35990892] (IHC, Rat) IHC Rat
M Mao, Y Xu, XY Zhang, L Yang, XB An, Y Qu, YN Chai, YR Wang, TT Li, J Ai MicroRNA-195 prevents hippocampal microglial/macrophage polarization towards the M1 phenotype induced by chronic brain hypoperfusion through regulating CX3CL1/CX3CR1 signaling J Neuroinflammation, 2020-08-20;17(1):244. 2020-08-20 [PMID: 32819407] (ICC, IHC, Rat) ICC, IHC Rat
S Castro-Sán, ÁJ García-Yag, T López-Royo, M Casarejos, JL Lanciego, I Lastres-Be Cx3cr1-deficiency exacerbates alpha-synuclein-A53T induced neuroinflammation and neurodegeneration in a mouse model of Parkinson&#039;s disease Glia, 2018-04-06;0(0):. 2018-04-06 [PMID: 29624735] (IHC, Mouse) IHC Mouse
Q.Q. Zhou, S.S. Chen, Q.Q. Zhang, P.F. Liu, H.Z. Fang, Y. Yang et al. Cerebrospinal fluid-contacting nucleus mediates nociception via release of fractalkine Brazilian Journal of Medical and Biological Research 8/7/2017 [PMID: 28793053]
José L M Madrigal Noradrenaline induces CX3CL1 production and release by neurons Neuropharmacology, 2016-12-05;114(0):146-155. 2016-12-05 [PMID: 27923568] (ICC, Western Blot, Rat) ICC, Western Blot Rat
Matthew K. Zabel, Lian Zhao, Yikui Zhang, Shaimar R. Gonzalez, Wenxin Ma, Xu Wang et al. Microglial phagocytosis and activation underlying photoreceptor degeneration is regulated by CX3CL1‐CX3CR1 signaling in a mouse model of retinitis pigmentosa Glia 9/1/2016 [PMID: 27314452]
Tomohiro Aoki, Kimiko Yamamoto, Miyuki Fukuda, Yuji Shimogonya, Shunichi Fukuda, Shuh Narumiya Sustained expression of MCP-1 by low wall shear stress loading concomitant with turbulent flow on endothelial cells of intracranial aneurysm Acta Neuropathologica Communications 12/1/2016 [PMID: 27160403]
Yeh P, Huang H, Yang C, Yang W, Yang C Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats. PLoS ONE, 2016-01-14;11(1):e0146438. 2016-01-14 [PMID: 26765843] (IHC-P, Western Blot, Rat) IHC-P, Western Blot Rat
Tejbeer Kaur, Darius Zamani, Ling Tong, Edwin W. Rubel, Kevin K. Ohlemiller, Keiko Hirose et al. Fractalkine Signaling Regulates Macrophage Recruitment into the Cochlea and Promotes the Survival of Spiral Ganglion Neurons after Selective Hair Cell Lesion The Journal of Neuroscience 11/11/2015 [PMID: 26558776]
Show All 17 Publications.

Reviews for CX3CL1/Fractalkine Antibody (AF537) (0)

There are no reviews for CX3CL1/Fractalkine Antibody (AF537). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CX3CL1/Fractalkine Antibody (AF537) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CX3CL1/Fractalkine Products

Blogs on CX3CL1/Fractalkine

There are no specific blogs for CX3CL1/Fractalkine, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CX3CL1/Fractalkine Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CX3CL1
Uniprot