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CRCP Antibody

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Western Blot: CRCP Antibody [NBP1-84495] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 10. Lane 2: Human cell line RT-4.
Western Blot: CRCP Antibody [NBP1-84495] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 10. Lane 2: Human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: CRCP Antibody [NBP1-84495] - Staining of human cell line A-431 shows localization to nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CRCP Antibody [NBP1-84495] - Staining of human testis shows cytoplasmic and nuclear positivity of cells in seminiferus ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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CRCP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TPCRHQSPEIVREFLTALKSHKLTKAEKLQLLNHRPVTAVEIQLMVEESEERLTEEQIEALLHTVTSILPAEPEAEQKKNTNSNVAMDEEDPA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRCP
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CRCP Protein (NBP1-84495PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CRCP Antibody

  • Calcitonin gene-related peptide-receptor component protein
  • CGRP receptor component
  • CGRPRCP
  • CGRP-RCPRNA polymerase III subunit C9
  • CGRP-receptor component protein
  • DNA-directed RNA polymerase III subunit RPC9
  • hsC17
  • RCP9
  • RCPMGC111194

Background

CRCP, also known as DNA-directed RNA polymerase III subunit RPC9, is a 17 kDa 148 amino acid protein with three shorter isoforms. The primary function of CRCP is to encode a protein which plays a role in increasing intracellular cAMP levels. CRCP is currently being studied in relation to cystic fibrosis, cryptorchidism, fibrosis, pancreatic cancer and pancreatitis. CRCP has been linked to the cellular localization, acrosome reaction, vasodilation, pathogenesis and parturition pathways where it interacts with POLD3, CHRAC1, VAV2, CALCRL and POLR3H.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CRCP
Entrez
Uniprot