![Immunohistochemistry-Paraffin: Chikungunya Virus nsp2 Antibody - Azide and BSA Free [NBP3-25408] - Chikungunya virus nsP2 antibody detects Chikungunya virus nsP2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and Chikungunya virus nsP2 - transfected 293T cell pellet. Green: Chikungunya virus nsP2 stained by Chikungunya virus nsP2 antibody (NBP3-25408) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25408_rabbit-chikungunya-virus-nsp2-pab-azide-and-bsa-free-2120249425494.jpg "Immunohistochemistry-Paraffin: Chikungunya Virus nsp2 Antibody - Azide and BSA Free [NBP3-25408] - Chikungunya virus nsP2 antibody detects Chikungunya virus nsP2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and Chikungunya virus nsP2 - transfected 293T cell pellet. Green: Chikungunya virus nsP2 stained by Chikungunya virus nsP2 antibody (NBP3-25408) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | Recombinant protein encompassing a sequence within the center region of Chikungunya Virus nsp2. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Chikungunya Virus nsp2 Antibody - Azide and BSA Free [NBP3-25408] - Chikungunya viral lysate (0.5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP2 antibody (NBP3-25408) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25408_rabbit-chikungunya-virus-nsp2-pab-azide-and-bsa-free-212024941441.jpg "Western Blot: Chikungunya Virus nsp2 Antibody - Azide and BSA Free [NBP3-25408] - Chikungunya viral lysate (0.5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP2 antibody (NBP3-25408) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: Chikungunya Virus nsp2 Antibody - Azide and BSA Free [NBP3-25408] - Chikungunya virus nsP2 antibody detects Chikungunya virus nsP2 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chikungunya virus nsP2 stained by Chikungunya virus nsP2 antibody (NBP3-25408) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25408_rabbit-chikungunya-virus-nsp2-pab-azide-and-bsa-free-2120249413342.jpg "Immunocytochemistry/Immunofluorescence: Chikungunya Virus nsp2 Antibody - Azide and BSA Free [NBP3-25408] - Chikungunya virus nsP2 antibody detects Chikungunya virus nsP2 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chikungunya virus nsP2 stained by Chikungunya virus nsP2 antibody (NBP3-25408) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")