Home » CEACAM5/CD66e » CEACAM5/CD66e Antibodies » CEACAM5/CD66e Antibody (SPM584) [Janelia Fluor® 549]
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CEACAM5/CD66e Antibody (SPM584) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
SPM584
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34851AF350
Alexa Fluor 405
NBP2-34851AF405
Alexa Fluor 488
NBP2-34851AF488
Alexa Fluor 532
NBP2-34851AF532
Alexa Fluor 594
NBP2-34851AF594
Alexa Fluor 647
NBP2-34851AF647
Alexa Fluor 700
NBP2-34851AF700
Alexa Fluor 750
NBP2-34851AF750
Allophycocyanin
NBP2-34851APC
Allophycocyanin/Cy7
NBP2-34851APCCY7
Biotin
NBP2-34851B
CoraFluor 1
NBP2-34851CL1
DyLight 350
NBP2-34851UV
DyLight 405
NBP2-34851V
DyLight 488
NBP2-34851G
DyLight 550
NBP2-34851R
DyLight 594
NBP2-34851DL594
DyLight 650
NBP2-34851C
DyLight 680
NBP2-34851FR
DyLight 755
NBP2-34851IR
FITC
NBP2-34851F
HRP
NBP2-34851H
Janelia Fluor 525
NBP2-34851JF525
Janelia Fluor 585
NBP2-34851JF585
Janelia Fluor 635
NBP2-34851JF635
Janelia Fluor 646
NBP2-34851JF646
Janelia Fluor 669
NBP2-34851JF669
PE
NBP2-34851PE
PE/Atto594
NBP2-34851PEATT594
PE/Cy5.5
NBP2-34851PECY55
PE/Cy7
NBP2-34851PECY7
PerCP
NBP2-34851PCP
Unconjugated
NBP2-33009-0.1mg
mFluor Violet 450 SE
NBP2-34851MFV450
mFluor Violet 500 SE
NBP2-34851MFV500
mFluor Violet 610 SE
NBP2-34851MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-33009-0.1mg
Azide and BSA Free
NBP2-34851-0.1mg
IHC-Prediluted
NBP2-44896
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CEACAM5/CD66e Antibody (SPM584) [Janelia Fluor® 549] Summary

Immunogen
Human colon carcinoma extract (Uniprot: P06731)
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This monoclonal antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CEACAM5/CD66e Antibody (SPM584) [Janelia Fluor® 549]

  • Carcinoembryonic antigen
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • CD66e antigen
  • CD66e
  • CEA
  • CEACAM5
  • CEACAM-5
  • CEACD66e
  • DKFZp781M2392
  • Meconium antigen 100

Background

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CEACAM5